Review on Identification of Proteins of Microorganisms by Two-dimensional gel electrophoresis- Mass spectrometry (2-DE-MS)

Mass spectrometry (MS) has greatly enhanced research in the field of microbial proteomics. Microbial characterization, including detection, differentiation, and identification, are the most important tasks of microbial proteomic laboratories worldwide.2-DE is a well-established technique for separation and quantitation of a large set of proteins and has much to contribute to the experimental analysis of microbial organisms. Proteins separated by 2-DE subsequently rely on MS techniques for identification and further characterization. This review will briefly summarize the basic concepts of 2-DE, and 2-DE/MS technology and its specific methods of detection of proteins of microorganisms, the proteome of cell membrane and intracellular or cytoplasamic proteome turnover in biomarker discovery. Clearly, it is not possible to cover all of the literatures that were published in these years. Therefore we focused on the success stories as well as some of the challenges that are currently being faced.